G - Physics – 01 – N
Patent
G - Physics
01
N
150/14, 150/3
G01N 33/48 (2006.01) C12Q 1/28 (2006.01) C12Q 1/54 (2006.01) C12Q 1/62 (2006.01) G01N 33/50 (2006.01)
Patent
CA 1100021
ABSTRACT OF THE DISCLOSURE Test means, such as a composition or device, method of making a test device and process for determination of at least one oxidizing substance, such as a peroxide, are disclosed. More particularly, the contemplated test means comprises a hydrazone and a hydroxynapthalenesulfonate selected from the group 4,5-dihydroxy-2,7-napthalene disulfonic acid (chromotropic acid) and 1-hydroxy-2-napthalene sulfonic acid. Further provided is a test system for the determination of a constituent in a sample, having means responsive to the presence of said constituent in said sample to produce at least one oxidizing substance and a composition for deter- mining said at least one oxidizing substance, having the improvement wherein said composition comprises a hydrazone and a hydroxynapthalenesulfonate selected from the group of 4,5-dihydroxy-2,7-napthalene disulfonic acid and 1-hydroxy- 2-napthalene sulfonic acid. The test system is preferably of the type which determines peroxides formed from enzymstic conversion of constituents in biological fluids. When in the form of compositions the test means can optionally be incorporated with a carrier, such as a tablet or matrix, to provide a test device. The indicator system is highly sensitive to low levels of body fluid constituents to be detected while also being highly resistant to interfering reducing substances, such as ascorbic acid, often present in body fluids.
312667
Miles Laboratories Inc.
Osler Hoskin & Harcourt Llp
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