High level expression in yeast

Details High level expression in yeast High level expression in yeast

C - Chemistry, Metallurgy

12

N

195/1.18, 195/1.

C12N 15/81 (2006.01) C07K 14/47 (2006.01) C07K 14/81 (2006.01) C12N 1/19 (2006.01) C12N 9/10 (2006.01) C12N 15/67 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1304020

HIGH LEVEL EXPRESSION IN YEAST Abstract of the Disclosure Methods for the enhanced production or proteins, such as alpha-1-antitrypsin, in yeast host cells are disclosed. The methods generally include introducing a suitable expression vector into a yeast host cell having a genetic defect. The expression vector is capable of directing the expression of heterologous genes or cDNA in yeast and contains an ADH2 pro- moter, a hoterologous gene or cDNA, and a detective selectable marker, the defective selectable marker complementing the genetic defect in the yeast host cell.

562260

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