Stable dna constructs for expression of alpha-1-antitrypsin

Details Stable dna constructs for expression of alpha-1-antitrypsin Stable dna constructs for expression of alpha-1-antitrypsin

C - Chemistry, Metallurgy

12

N

195/1.235, 195/1

C12N 15/00 (2006.01) C07G 99/00 (2009.01) C07H 21/04 (2006.01) C12N 1/00 (2006.01) C12N 1/16 (2006.01) C12N 1/19 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 5/00 (2006.01) C12N 9/99 (2006.01) C12N 15/09 (2006.01) C12N 15/15 (2006.01) C12N 15/64 (2006.01) C12P 19/34 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1299120

ABSTRACT Methods are provided for producing alpha-1-antitrypsin in host cells and for selecting transformed cells comprising the step of transforming the host cell with a DNA molecule comprising a gene which complements a deficiency in the host cell. The host cell is a strain having a deficiency in a function necessary for normal cell growth. The gene in the DNA molecule, such as a plasmid, which complements the deficiency serves as a selectable marker whereby the growth conditions for selection may comprise a conventional complex medium.

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