Methods of assay

Details Methods of assay Methods of assay

G - Physics

01

N

167/44

G01N 33/537 (2006.01) G01N 33/538 (2006.01) G01N 33/563 (2006.01) G01N 33/577 (2006.01) G01N 33/74 (2006.01)

Patent

CA 1261745

ABSTRACT METHODS OF ASSAY The present invention provides a method of assay of a ligand in a sample, which comprises incubating, simultaneously or in sequence, (a) the sample (containing the analyte ligand), (b) a directly labelled specific binding partner to the ligand, (c) a ligand analogue as herein defined, conjugated with a reagent X (the said reagent not being present as a free reagent in the mixture); separating, after a suitable incubation period, the portion containing component (c) from the mixture by means of a solid phase carrying a binding partner specific for reagent X and determining the extent of complexing between components (b) and (c) by measurement of the label.

490004

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