Latex agglutination immunoassay in the presence of hemoglobin

Details Latex agglutination immunoassay in the presence of hemoglobin Latex agglutination immunoassay in the presence of hemoglobin

G - Physics

01

N

167/44

G01N 33/546 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/577 (2006.01) G01N 33/72 (2006.01)

Patent

CA 1332043

ABSTRACT OF THE DISCLOSURE A latex agglutination immunoassay method for determining an analyte in a blood sample in which the pH of the reaction mixture is maintained at about 8.5 or greater in order to overcome nonspecific agglutination of latex particles by hemoglobin present in the sample. The method is particularly applicable to the determination of glycated hemoglobin, e.g., Hb Alc. In another embodiment, native hemoglobin (Ao) can be determined based on its ability to cause agglutination of latex particles suspended in an aqueous solution having a pH of about 8 or below.

576067

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