Liver assist device employing transformed cell lines

Details Liver assist device employing transformed cell lines Liver assist device employing transformed cell lines

A - Human Necessities

61

M

167/327, 195/46

A61M 1/16 (2006.01)

Patent

CA 1287314

LIVER ASSIST DEVICE EMPLOYING TRANSFORMED CELL LINES Abstract of the Disclosure An improved extracorporeal liver assist device and method is provided which employs a blood perfusion membrane cultured with initially trans- formed hepatocytes until a confluent monolayer is developed, whereupon the hepatocytes are reverted to the somatic phenotype for perfusion purposes. Use of transformed hepatocytes permits serial subculturing to maintain a clinical supply of cells for the patient, while the in vitro proliferation characteristics and loss of contact inhibition of the transformed hepatocytes ensures rapid cell division and layer formation on the perfusion membranes. Virally transformed, temperature sensi- tive hepatocytes are preferred so that reversion of the cells can be accomplished by temperature change. The transformed hepatocytes may be cul- tured on the exterior surfaces of multiple capil- lary membrane cartridges, and subsequently reverted by elevating the temperature thereof. During perfusion, the patient's blood is passed through the lumen of the capillaries, and dissolved mole- cular species (e.g., bilirubin) diffuse through the membrane to be taken up and metabolized by the hepatocytes. Bathing solution is simultaneously passed around the exterior of the capillary tubes to remove metabolic wastes from the hepatocytes.

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