Immobilized nucleic acid-containing probes

Details Immobilized nucleic acid-containing probes Immobilized nucleic acid-containing probes

C - Chemistry, Metallurgy

12

Q

150/3, 195/1.12,

C12Q 1/68 (2006.01) G01N 33/548 (2006.01)

Patent

CA 1223222

ABSTRACT An immobilized nucleic acid-containing probe suitable for accurate hybridization gene analyses is produced by enzymatically coupling a nucleotide to a predetermined end of the nucleic acid and then selective- ly coupling a suitably prepared solid support, such as cellulose, through the nucleotide. Thus the ribose of the nucleotide can be oxidized by sodium periodate to form aldehyde groups which will react with a primary amino group introduced into cellulose, forming a Schiff's base. For greater stability of the immobilizing bond the Schiff's base is reduced to a saturated secondary amino group by reduction with borohydride. Alternatively, the nucleotide may carry an SH or Hg moiety which will selectively react with complementary moieties on the solid support.

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