Use of synthetic bifunctional ligand for the immunometric...

Details Use of synthetic bifunctional ligand for the immunometric... Use of synthetic bifunctional ligand for the immunometric...

G - Physics

01

N

167/44

G01N 33/50 (2006.01) G01N 33/54 (1980.01)

Patent

CA 1183080

Abstract The use of a synthetic bifunctional ligand for the immunometric determination of the concentration ratio of two solutes The specification describes an immunochemical method for detecting the fertile period of the menstrual cycle, by determining the ratio, in urine of the two hormonal metabolites oestrone-3-glucuronide (E13G) and pregane- diol-3-glucuronide (PD3G). The method depends of deter- mining how an immunocomplex dissociates in the presence of the two metabolites. The immunocomplex comprises a solid phase with antibodies to PD3G attached, and bifunctional ligand (E13G and PD3G both covalently bound to bovine serum albumin) bound to these antibodies, finally E13G antibodies are bound to the E13G moieties of the bifunctional ligand. The immunocomplex is incubated with urine. The meta- bolites in the urine compete for the antibodies so the immunocomplex partially dissociates. The solid phase is washed and the number of exposed E13G moieties remaining depends on the ratio of E13G to PD3C in the urine. These are determined with enzyme labelled E13G antibodies.

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