Immobilization of enzymes

Details Immobilization of enzymes Immobilization of enzymes

C - Chemistry, Metallurgy

12

N

195/35

C12N 11/04 (2006.01)

Patent

CA 1277267

Abstract of the Disclosure: Enzymes are immobilized by a process in which the following components are mixed with one another in aqueous solution: a) an enzyme or a dispersed microorganism, b) a water-soluble glycidyl ether, prepared by subjecting from 1 to 30 equivalents of an epoxide of the formula I Image ( I ) where R1, R2 and R3 are each hydrogen, methyl or ethyl, to an addition reaction with 1 equivalent (based on OH groups) of a polyfunctional alcohol of 2 to 6 car- bon atoms or of a mono- or disaccharide, reacting the resulting adduct with epichlorohydrin and, if required, then carrying out cyclization to give the epoxide, and c) a water-soluble amine possessing two or more NH groups, if necessary in a partially neutralized form, and the mixture thus obtained is allowed to gel.

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