Immunoassay

Details Immunoassay Immunoassay

G - Physics

01

N

167/38, 167/103.

G01N 33/573 (2006.01) C07J 17/00 (2006.01) C07J 41/00 (2006.01) C07J 43/00 (2006.01) G01N 33/74 (2006.01)

Patent

CA 1223812

ABSTRACT: IMMUNOASSAY There is described an enzyme immunoassay for measuring the concentration of an analyte in a sample wherein the substrate for the enzyme forms at least a part of the sample. In a particular embodiment the sample comprises or consists of a milk sample and the enzyme is an enzyme capable of clotting milk. An example given of such an enzyme is chymosin. The assay described may be used to measure the concentration of progestagens or oestrogens in milk using the techniques of heterogen- eous or homogeneous enzyme immunoassay. The results of such an assay give an indication of the fertility of a milk producing domestic animal (e.g. a cow) and may be used to diagnose pregnancy of such an animal. Particular compounds for use in the assay are described, as is a kit of reagents for use in the assay.

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