C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
167/43, 150/15.3
C12N 9/64 (2006.01) C12Q 1/37 (2006.01) G01N 33/564 (2006.01) G01N 33/569 (2006.01) G01N 33/573 (2006.01)
Patent
CA 1265742
ABSTRACT A reagent and process for the detection of the presence of an allergy and for the specific detection of the allergen responsible for the allergy, in which the leukocytes of a sample to be investigated are incubated with an allergen or with another stimu- lation factor in an aqueous medium together with a chromogenic protease substrate and calcium ions; the liberated protease is reacted with a chromogen and the resulting chromophor is determined; in particular, the allergen is mixed in a buffer with a pH of from 6.3 to 9 with 0.5 to 5 x 10 -3 mol/l. of calcium ions and 10-4 to 10-3 mol/l. of chromogen in isotonic solution and mixed with 109 to 1010 of the leukocytes to be investigated per litre and the protease activity is measured kinetically after an incubation period by the increase of the chromophor concentration; the process may be employed for the determination of antiallergic and anti-inflammatory substances.
497664
Boehringer Mannheim Gmbh
Swabey Ogilvy Renault
LandOfFree
Process for the detection of the presence of an allergy and... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for the detection of the presence of an allergy and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for the detection of the presence of an allergy and... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1217520