Method of producing a factor viii (ahf) containing fraction

Details Method of producing a factor viii (ahf) containing fraction Method of producing a factor viii (ahf) containing fraction

A - Human Necessities

61

K

167/103.34

A61K 35/16 (2006.01) C07K 14/755 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1297011

ABSTRACT OF THE DISCLOSURE: There is disclosed a method of producing a Factor VIII (AHF) containing fraction having a specific activity of at least 2.5 units of Factor VIII/mg protein as well as a portion of immunoglobulin G (IgG) of 10 mg/1000 units of Factor VIII at the most. Its risk of transmission of viral or bacterial infections is to be avoided or largely reduced. The method consists in that undesired proteins are at first precipitated from a Factor VIII (AHF) containing plasma fraction in the presence of SPS. The purified Factor VIII containing solution is treated with suitable salts or salt mixtures in order to obtain a Factor VIII containing precipitate. This precipitate is dissolved, lyophilized and finally heat-treated.

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