Phosphorylating reagents and method of using the same

Details Phosphorylating reagents and method of using the same Phosphorylating reagents and method of using the same

C - Chemistry, Metallurgy

07

F

260/229, 260/462

C07F 9/141 (2006.01) C07F 9/09 (2006.01) C07F 9/146 (2006.01) C07F 9/24 (2006.01) C07H 21/00 (2006.01)

Patent

CA 1300051

-31- PHOSPHORYLATING REAGENTS AND METHOD OF USING THE SAME ABSTRACT OF THE DISCLOSURE Novel phosphorylating reagents are provided which are useful in DNA synthesis and purification procedures. The reagents are substituted phosphines which are particularly useful in the phosphorylation of nucleoside and solid supported oligonucleotides at the 5'- hydroxyl position. Phosphorylation using these reagents is easily and accurately monitored colorimetrically.

573448

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