C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
47/4, 195/47, 47
C12N 15/84 (2006.01) A01H 1/04 (2006.01) A01H 4/00 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01)
Patent
CA 1335799
A method for transforming cotton. A cotton explant is contacted with Agrobacterium vector containing an expressible gene code foreign to cotton sufficient to transfer the gene to the explant. The explant is then incubated on a first solid callus growth medium containing glucose as the carbon source for about 15 to about 200 hours at a defined temperature under defined light and dark cycles. The incubated explants is then contacted with a first solid callus grown medium as a carbon source and an antibiotic toxic to the Agrobacterium to kill the Agrobacterium. Further culturing the explant containing the transferred gene on a first solid callus growth medium containing glucose as the carbon source and free of bacterium until phenolic secretions from the explant forming the callus end and for undifferentiated callus to develop. During said development of the undifferentiated callus, callus selecting transformed callus from untransformed callus and transferring the selected transformed callus to a second solid growth medium containing sucrose as the carbon source.
616835
Anderson David Maurice
Grula John William
Hudspeth Richard Lorne
Rajasekaran Kanniah
Rangan Thirumale Srinivasa
Fetherstonhaugh & Co.
Mycogen Corporation
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