Transfection high voltage controller

Details Transfection high voltage controller Transfection high voltage controller

A - Human Necessities

61

N

323/10

A61N 1/00 (2006.01) A61N 1/18 (2006.01) B01J 19/08 (2006.01) C12M 3/00 (2006.01) C12N 13/00 (2006.01) C12N 15/87 (2006.01) H03K 3/57 (2006.01) H03K 17/10 (2006.01)

Patent

CA 1326507

23 TRANSFECTION HIGH-VOLTAGE CONTROLLER ABSTRACT OF THE DISCLOSURE A transfection high-voltage controller for directing high-voltage currents to a suspension of cells and DNA comprising a control section, a power store, and a high joule switch. Under control of the control section, the high joule switch directs a high-voltage current from the power store to a load such as a cuvette. The high-joule switch comprises a semiconductor con- trolled rectifier (SCR) having a gate coupled to the control unit for triggering the device, an anode for receiving the high-voltage current from the power store, and a cathode for delivering the high-voltage current to the cuvette. A current sink may be connected to the anode of the SCR to render it non-conductive a predeter- mined time after the high-voltage current appears at the cathode. Multiple SCRs may be connected in series to support voltages over 3,000 and currents in excess of 125 amperes, and a trigger control unit may be attached to the gate of each SCR in the series to effect simultaneous or serial triggering of the group.

538821

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