Biological indicator for sterilization processes

Details Biological indicator for sterilization processes Biological indicator for sterilization processes

C - Chemistry, Metallurgy

12

Q

150/16.2

C12Q 1/22 (2006.01) G01N 31/22 (2006.01)

Patent

CA 1174152

ABSTRACT A biochemical test system is disclosed for use in a biolo- gical fermentation sterilization indicator. The carbohydrate component of the nutrient broth media is removed from the media. The carbohydrate component may be disposed in the cavity of an encapsulated sealed glass ampul which contains a non-carbohy- drated test solution. Microorganism spores are also disposed in the cavity. Upon completion of sterilization the ampul is broken; the contents mix. Fermentation of viable spores yields growth and color change and is a positive test (unsatisfactory sterilization); no fermentation yields no growth, no color change and satisfactory sterilization. The separation of carbo- hydrate prevents non-microorganism fermentation on the test reaction, and the appearance of a "false positive".

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