Method and kit for detecting polyriboadenosine segments and...

Details Method and kit for detecting polyriboadenosine segments and... Method and kit for detecting polyriboadenosine segments and...

C - Chemistry, Metallurgy

12

Q

150/8.5

C12Q 1/68 (2006.01) C12Q 1/34 (2006.01) C12Q 1/48 (2006.01)

Patent

CA 1259555

ABSTRACT METHOD AND KIT FOR DETECTING POLYRIBOADENOSINE SEGMENTS AND MESSENGER RNA RNA such as messenger RNA is digested to nucleotide phosphates including AMP or ADP. The ATP or a byproduct of the osphorylation, e.g., pyruvate, is detected. Exemplary enzymes d (with appropriate co-reactants and co-factors) are: (1) polynucleotide phosphorylase, pyruvate kinase and luciferase, or 2) phosphodiesterase (or RNase), myokinase, pyruvate kinase and luciferase. The phosphorylation to ATP (e.g., with pyruvate kinase) is preferably coupled with the previous (reversible) enzymatic step.

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