Preparation of functional human factor viii

Details Preparation of functional human factor viii Preparation of functional human factor viii

C - Chemistry, Metallurgy

07

K

530/13, 167/103.

C07K 14/755 (2006.01) A61K 38/37 (2006.01) C12N 15/10 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1341395

The full DNA coding sequence of human factor VIII is identified herein. Also disclosed is the recombinant means useful to isolate and express this coding sequence in the preparation of functional human factor VIII polypeptide and functional derivatives thereof. Particularly preferred embodiments provide proof of unequivocal identification and functionality as well as specific, operable recombinant expression vectors and cultures. This invention marks the successful application of recombinant DNA technology in the preparation of the largest and most elusive of human proteins, a goal with technical problems probably insurmountable to workers of ordinary skill having mere possession, e.g., of a partial or full clone encoding factor VIII.

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