Isolation of bioactive, monomeric growth hormone

Details Isolation of bioactive, monomeric growth hormone Isolation of bioactive, monomeric growth hormone

C - Chemistry, Metallurgy

12

P

195/72, 195/1.32

C12P 21/02 (2006.01) C07K 1/113 (2006.01) C07K 14/61 (2006.01)

Patent

CA 1273592

ABSTRACT Monomeric, biologically active growth hormone is isolated from microbially-produced insoluble inclusion bodies by solubilizing and denaturing the growth hormone by extraction of the inclusion bodies into a guanidine salt solution such as guanidine hydrochloride and subsequently renaturing at least a portion of the growth hormone in the solution by replacing the guanidine salt solution with a denaturant-free buffer solution and removing precipitated impurities and growth hormone aggregates. The renatured growth hormone is then purified by ion-exchange chromatography.

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