Stable high copy number plasmids

Details Stable high copy number plasmids Stable high copy number plasmids

C - Chemistry, Metallurgy

12

N

195/1.19, 195/1.

C12N 15/00 (2006.01) C12N 15/69 (2006.01) C12N 15/70 (2006.01) C12P 21/00 (2006.01)

Patent

CA 1198067

STABLE HIGH COPY NUMBER PLASMIDS ABSTRACT DNA plasmids are described which are selected mutants in which an altered repressor gene leads to high copy number replication. elements in the plasmids are modified by placing a suitable transcriptional "stop" signal after an inserted heterogenous gene so that readthrough expression of heterologous DNA inserted in the plasmid will not continue into the replication primer strand. Deletions resulting from interference with replication primer strand transcription are thereby avoided.

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