Hybrid promoter, expression controlling dna sequence and...

Details Hybrid promoter, expression controlling dna sequence and... Hybrid promoter, expression controlling dna sequence and...

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.2

C12N 15/73 (2006.01) C12N 15/70 (2006.01)

Patent

CA 1309042

- 17 - ABSTRACT: The present invention provides novel hybrid promoters comprising an RNA polymerase recognition region having a base sequence equivalent to that of the RNA polymerase recognition region of a phage promoter, and an RNA polymerase binding region having a base sequence equivalent to that of the RNA polymerase binding region of an E. coli promoter. Disclosed also are expression- controlling DNA sequences comprising said hybrid promoter and an operator region having a base sequence equivalent to that of the operator region of E. coli, which allow for efficient expression in a well-controlled manner. Disclosed further are expression vectors comprising a terminator and the gene encoding a target protein as well as said expression-controlling DNA sequence.

551524

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