Homogeneous specific binding assay element and method for...

Details Homogeneous specific binding assay element and method for... Homogeneous specific binding assay element and method for...

G - Physics

01

N

167/45

G01N 33/58 (2006.01) G01N 33/542 (2006.01)

Patent

CA 1179262

ABSTRACT A homogeneous specific binding assay element, a method for its preparation, and a method for its use in determining a ligand in or the ligand binding capacity of a liquid sample are disclosed. The test element comprises a solid carrier incorporated with reagents for a homogeneous specific binding assay system which produces a detectable response, usually an electromagnetic radiation signal, that is a func- tion of the presence or amount of the ligand in or the ligand binding capacity of the sample. There is further disclosed a method for preparing a homo- geneous specific binding assay element for deter- mining a ligand in or the ligand binding capacity of a liquid sample by incorporating a carrier with a composition which includes a label conjugate, com- prising a label component coupled to a ligand moiety or a specific binding analog thereof, and a reagent reactive with the label conjugate, which method comprises (a) incorporating the carrier with the reagent reactive with the label conjugate in a first liquid and drying the carrier; and then (b) incorporating the carrier of (a) with the label conjugate in a liquid effective to prevent reaction with the reagent reactive with the label conjugate prior to contact of the element with the sample and drying the carrier.

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