Process for the extraction and purification of proteins from...

C - Chemistry – Metallurgy – 12 – P

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C12P 21/02 (2006.01) C07K 1/36 (2006.01) C07K 14/02 (2006.01) C12N 1/06 (2006.01) C12N 15/00 (2006.01)

Patent

CA 1272457

TITLE Process for the extraction and purification of proteins from culture media producing them ABSTRACT The process is applicable to the supernatant of engineered yeast cells disrupted in the presence of a non-ionic detergent: it comprises the precipitation of contaminants by polyethylene glycol and the treatment of this latter supernatant with either a bivalent metal cation or, after eventual ultrafiltration, with ammo- nium sulfate.

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