Solid-phase non-separation enzyme assay

Details Solid-phase non-separation enzyme assay Solid-phase non-separation enzyme assay

G - Physics

01

N

167/45

G01N 33/577 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01) G01N 33/563 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1335173

Novel assays employing enzyme fragments which complex to form an active enzyme are provided. The reagents involved are a member of a specific binding pair bound to a solid surface, a first enzyme fragment conjugated to a member of a specific binding pair complementary or cross-reactive in relation to the analyte or antianalyte and a second enzyme fragment which binds to the conjugate to form an active enzyme, where the first enzyme fragment conjugate may be present in up to substantial excess to ensure at least substantially complete binding of all of the analyte. By distributing the conjugate between the solid surface and the medium in relation to the amount of analyte present, the enzyme activity may be determined in relation to the amount of analyte present.

577915

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