Recombinant 2,5-diketogluconic acid reductase production

C - Chemistry – Metallurgy – 12 – N

Patent

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195/114, 195/129

C12N 15/53 (2006.01) C12N 9/04 (2006.01) C12P 7/60 (2006.01)

Patent

CA 1341085

The invention relates to purification of and recombinant production of 2.5-diketogluconic acid (2.5-DKG) reductase and the use of the reductase so produced in converting 2.5-DKG stereoselectively into 2-keto-L-gluconic acid (2 KLG), as well as to the production of a single recombinant organism capable of synthesizing 2-KLG. The 2-KLG produced is a useful intermediate in the production of ascorbic acid (vitamin C).

590942

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