G - Physics
01
N
167/44
G01N 33/543 (2006.01) C07K 14/56 (2006.01) G01N 33/68 (2006.01)
Patent
CA 1288692
ABSTRACT OF THE DISCLOSURE A rapid assay for quantifying the amounts of interferon in liquid samples is provided. The assay employs an affinity column, containing immobilized anti-interferon antibodies, in series with a reversed-phase column. The assay is able to measure interferon concentrations as low as 1-2 micrograms/milliliter in total assay times of less than one hour. By suitably adjusting the elution gradient used with the reversed-phase column, different interferon subtypes, e.g., monomeric and oligomeric forms of .alpha.2-interferon, can be separately detected and quantified. In certain preferred embodiments, errors due to non-speciic binding are minimized by treating the affinity column with an albumin-contalning buffer prior to each assay.
549264
D'andrea Mark J.
Rybacek Ludvik
Tarnowski Stanley J. Jr.
D'andrea Mark J.
Interferon Sciences Inc.
Rybacek Ludvik
Sim & Mcburney
Tarnowski Stanley J. Jr.
LandOfFree
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