Method and element for albumin assay

Details Method and element for albumin assay Method and element for albumin assay

G - Physics

01

N

150/14, 150/3

G01N 33/68 (2006.01)

Patent

CA 1217697

-0- METHOD AND ELEMENT FOR ALBUMIN ASSAY ABSTRACT OF THE DISCLOSURE Disclosed herein is an assay for determination of albumin in a liquid sample. This assay involves the use of a dye of the structure: Image wherein n is zero or a positive integer up to 3; R1, R2 and R3 are independently hydrogen, alkyl, cycloalkyl, aryl, alkoxy, aroxy, hydroxy, carboxy, alkoxycarbonyl, amino or a nonaromatic heterocyclic group; and Z and Z1 independently represent the carbon, nitrogen, oxygen or sulfur atoms needed to complete a substituted or unsubstituted 5- to 7-membered carbocyclic or heterocyclic ring. This dye has a high affinity for albumin over a wide pH range. When the dye is bound to albumin, a shift in the dye spectral absorption occurs. Analytical elements containing this dye and an assay method are disclosed herein.

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