Process for the isolation and separation of lysozyme and...

Details Process for the isolation and separation of lysozyme and... Process for the isolation and separation of lysozyme and...

C - Chemistry, Metallurgy

12

N

195/39.1, 530/3.

C12N 9/36 (2006.01) C07K 14/465 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1283072

ABSTRACT A process for the isolation and separation of lysozyme and avidin from egg white which comprises: step a) contacting egg white with a weakly acidic cation exchange resin whereby lysozyme and avidin are adsorbed on to the resin; separating the resin from the egg white and washing the resin to remove residual egg white therefrom; and contacting the washed resin with a low ionic strength eluting buffer whereby lysozyme is eluted from the resin while avidin remains adsorbed on the resin; and step b) repeating the complete procedure defined in step a) for two or more times; and step c) finally contacting the resin containing ac- cumulated adsorbed avidin with a high ionic strength eluting buffer whereby avidin is eluted from the resin. Lysozyme and avidin are both commercial products use- ful, for example, in pharmaceutical applications.

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