C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.36
C12N 5/10 (2006.01) C12N 9/72 (2006.01) C12N 15/67 (2006.01) C12N 15/85 (2006.01)
Patent
CA 1304026
ABSTRACT OF THE DISCLOSURE Disclosed are vectors for achieving high level expression in eucaryotic cells. The vectors include an expressible gene encoding a protein product of interest, an expressible gene encoding a marker protein which permits selection of useful transformants, and an enhancer element, preferably a cellular enhancer element, which functions to increase the level of transcription of genes disposed on its 3' and 5' sides. A blocking element is interposed between the enhancer element and the marker gene which shields the promoter of the marker gene from the transcription-stimulating function of the enhancer, thereby limiting the effect of the enhancer to transcriptions of the DNA encoding the protein product of interest. Use of the vectors permits isolation of viable clones characterized by a very high level of expression of the protein of interest.
526756
Damon Biotech Inc.
Riches Mckenzie & Herbert Llp
LandOfFree
Vector and method for achieving high level expression in... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Vector and method for achieving high level expression in..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Vector and method for achieving high level expression in... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1262995