Process for recovering refractile bodies containing...

Details Process for recovering refractile bodies containing... Process for recovering refractile bodies containing...

C - Chemistry, Metallurgy

07

K

530/15, 167/103.

C07K 14/55 (2006.01) C07K 1/113 (2006.01) C07K 1/36 (2006.01) C07K 14/565 (2006.01)

Patent

CA 1329308

Abstract of the Disclosure A refractile material containing a heterologous protein is recovered from a host microorganism cell culture transformed to produce the protein. One recovery process involves disrupting the cell wall and membrane of the host cell, removing greater than 99% by weight of the salts from the disruptate, redisrupting the desalted disruptate, adding a material to the disruptate to create a density or viscosity gradient in the liquid within the disruptate, and separating the refractile material from the cellular debris by high-speed centrifugation. Another version of such a recovery process comprises the further steps of solubilizing the refractile material under reducing conditions, organically extracting the solubilized refractile material, and isolating said refractile material from the extractant. Preferably the protein is recombinant IL-2 or IFN-.beta. and the salt removal step is carried out by diafiltration.

507016

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