Bilirubin assay

Details Bilirubin assay Bilirubin assay

G - Physics

01

N

150/3, 150/9

G01N 33/72 (2006.01) G01N 33/92 (2006.01)

Patent

CA 1182724

ABSTRACT OF THE DISCLOSURE An improved reagent and method for assay of total bilirubin in biological fluids utilizes diazo sulfanilic acid to convert bilirubin to azobilirubin which is then quantitatively measured. The improved reagent and method include the solvent accelerator tetramethylene sulfone. The use of tetramethylene sulfone allows for the use of nonionic suriactants, obviating the need for serum blank correction in highly lipemic serum. Furthermore, the use of tetramethylene sulfone and nonionic surfactants renders the reagent mixsture less viscous facilitating automated analysis.

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