Method and apparatus for the determination of substances in...

Details Method and apparatus for the determination of substances in... Method and apparatus for the determination of substances in...

G - Physics

01

N

324/28.2

G01N 27/32 (2006.01) G01N 27/416 (2006.01) G01N 33/487 (2006.01) G01N 33/84 (2006.01)

Patent

CA 1174731

ABSTRACT OF THE DISCLOSURE A method and apparatus for the determination of the concentration of certain chemical substances such as glucose, urea, enzymes, etc, in biological solutions such as blood, serum, urine using a technique of differential pH measurement using two pH electrodes, and measuring pH changes in the solutions after the addition of specific reagents, the concentration being determined according to the formula [sample] = FCAL x (.DELTA.pHc - .DELTA.pHb - .DELTA.pHo) wherein [sample] is the concen- tration of the substance in the sample, FCAL is a calibration factor, .DELTA.pHc is the measure pH of the reacted sample, .DELTA.pHb is a pH correction for the reaction initiating compound, and .DELTA.pHo is a correction for background pH changes.

386932

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