Asporogenic mutant of bacillus subtilis useful as a host in...

C - Chemistry – Metallurgy – 12 – N

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195/1.4, 195/34.

C12N 1/20 (2006.01) C12N 15/75 (2006.01)

Patent

CA 1191100

ABSTRACT A new mutant of B. subtilis DE100 (ATCC 39,094) suitable for use as a host component in a host-vector system into which vectors containing various genes can be inserted. The mutant is characterized by having a frequency of reversion to spore formers of less than about 10-7 when grown under conditions of aeration and by having the following genetic markers: thy A1, thy B1, trp C2, pyr D1, and spo OA.DELTA.677.

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