Expression plasmids for improved production of heterologus...

Details Expression plasmids for improved production of heterologus... Expression plasmids for improved production of heterologus...

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.2

C12N 15/70 (2006.01) C07K 14/485 (2006.01) C07K 14/61 (2006.01) C12N 1/21 (2006.01) C12N 9/50 (2006.01) C12N 9/64 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1340867

Promote-ribosome binding site (rbs) expression elements of general utility for high level heterologous gene expression; plasmids carrying said promoter-rbs expression elements and encoding genetic information for direct high level expression in bacteria of heterologous proteins, especially plasmids carrying a gene coding for prorennin or mammalian growth hormones; methods for their construction, including the use of synthetic linkers to provide desirable functional properties thereto; recombinant microorganisms comprising said plasmids; expression of said bacterial produced heterologous proteins by said recombinant microorganisms; and demonstration of the activities of the thus-produced proteins.

470825

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