Silver stain for rapid, quantitative detection of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G01N 1/30 (2006.01) G01N 27/447 (2006.01) G01N 33/52 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1294519

Silver Stain for Rapid, Quantitative Detection of Polypeptides and Nucleic Acids Abstract A simple, positive image forming silver stain which takes less than 15 minutes to perform is disclosed for the detection of nanogram quantities of proteins and DNA on membranes and thin layer plates. This stain demonstrates a reproducible curvilinear relationship between silver density and the amount of protein or DNA, over an averaged concentration range from 1 nanogram to 300 nanograms for proteins and 10 nanograms to 700 nanograms for DNA. The ease of staining proteins and DNA on membranes, combined with the stain's sensitivity and reproducibility, permits quantitative determination and assay of proteins and DNA.

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