Process for the preparation of polypeptides utilizing a...

Details Process for the preparation of polypeptides utilizing a... Process for the preparation of polypeptides utilizing a...

C - Chemistry, Metallurgy

12

N

195/1.29

C12N 15/00 (2006.01) C07K 14/485 (2006.01) C12P 21/02 (2006.01) C12Q 1/37 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1195627

ABSTRACT A method for the production of polypeptides utilizing a polymer of charged amino acids and an exopeptidase. A DNA sequence coding for the charged amino acid polymer is inserted adjacent to one end of the polypeptide gene. The polypeptide-charged amino acid polymer fusion product is removed from most contaminents by selecting for the charged amino acid polymer during a first isolation step. Next the charged amino acid polymer is hydrolyzed using an exopeptidase which leaves the polypeptide active. A second isolation step removes the polypeptide from any remaining contaminents. An example using polyarginine and carboxypeptidase B to produce urogastrone is included.

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