C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
167/139, 195/46
C07K 14/52 (2006.01) A61K 35/12 (2006.01) A61K 38/00 (2006.01)
Patent
CA 1199579
- 27- Abstract of the Disclosure: A process is provided for the purification of a proteinaceous physiologically active substance having antitumor activity, which is induced by administering to a rabbit at least one substance having a capacity for stimulating reticuloendothelial system and then injecting endotoxin from a Gram-negative bacterium into the rabbit. The process comprises contacting a crude solution of said proteinaceous physiologically active substance with a basic anion exchanger to have said physiologically active substance adsorbed on the anion exchanger, eluting the adsorbed physiologically active substance, and subjecting the eluate containing said physiologically active substance to gel filtration with a gel suitable for separation of a substance with a molecular weight in the range of 30,000 to 70,000. The purified preparation of said physiologically active substance thus obtained may be used as an antitumor agent for the treatment of malignant tumors. Said physiologically active substance is isolated by subjecting the purified preparation successively to affinity chromatography on immobilized Cibacron Blue F3G-A, gel filtration, affinity chromato- graphy on immobilized concanavalin A, preparative electrophoresis on polyacrylamide gel and gel filtration.
405748
Haranaka Katsuyuki
Old Lloyd J.
Richards Elizabeth C.
Williamson Barbara
Asahi Kasei Kogyo Kabushiki Kaisha
Dainippon Pharmaceutical Co. Ltd.
Kirby Eades Gale Baker
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