C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.22, 195/1.
C12N 15/81 (2006.01) C07K 14/415 (2006.01) C12N 15/11 (2006.01) C12N 15/54 (2006.01) C12N 15/67 (2006.01)
Patent
CA 1333162
A method of expressing genes in yeast by introducing into a yeast cell a recombinant DNA segment con- taining both the gene to be expressed and a 5' flanking region comprising a promoter sequence, and culturing of the transformed yeast cells in a growth medium, using as the recombinant DNA segment a seg- ment, of which the 5' flanking region comprises a first DNA fragment containing a promoter sequence in combination with a second DNA fragment containing a leader sequence regulated on the posttranscrip- tional level by heme, heme analogs, or heme pre- cursors. Furthermore DNA-fragments and plasmids to be used when carrying out the method. When carrying out the method according to the invention an in- creased expression of genes is obtained by a reduced genetic load of the host cell and a more optimal utilization of the protein synthesis apparatus and the energy metabolism.
521391
Jensen Erik Õstergard
Marcker Kjeld Adrian
Danisco A/s
Macrae & Co.
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