Acid-labile sub-unit (als) of insulin-like growth factor...

Details Acid-labile sub-unit (als) of insulin-like growth factor... Acid-labile sub-unit (als) of insulin-like growth factor...

C - Chemistry, Metallurgy

07

K

150/9, 195/1.112

C07K 14/47 (2006.01) A61K 38/17 (2006.01) C07K 1/22 (2006.01) C07K 14/65 (2006.01) C07K 16/18 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1341598

The acid-labile sub-unit (ALS) of insulin like growth factor binding protein complex in biologically pure form is described. ALS has a molecular weight between 80-115kd as determined by SDS polyacrylamide gel electrophoresis, run under reducing conditions; and a partial N-terminal amino acid sequence as follows: Gly AspProGlyThrProGlyGluAlaGluGlyProAlaCysProAlaAlaCys-- Ala wherein the first amino acid may be Gly or Ala. Also described are methods of producing ALS, compositions containing the in-vivo IGF protein complex, methods of detecting ALS in body fluids, recombinant nucleic acid sequences encoding ALS, and expression vectors and host cells containing such nucleic acid sequences.

605669

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