Process for recovering refractile bodies containing...

Details Process for recovering refractile bodies containing... Process for recovering refractile bodies containing...

C - Chemistry, Metallurgy

12

N

195/73, 530/3.12

C12N 1/06 (2006.01) C07K 1/113 (2006.01) C07K 14/53 (2006.01) C07K 14/55 (2006.01) C12N 15/19 (2006.01)

Patent

CA 1329373

PROCESS FOR RECOVERING REFRACTILE BODES CONTAINING HETEROLOGOUS PROTEINS FROM MICROBIAL HOSTS ABSTRACT A refractile material containing a heterologous protein is recovered from a host microorganism cell culture transformed to produce the protein. One recoveryprocess involves first reducing the ionic strength of the culture medium prior to disruption to a level effective in preventing reaggregation of cellular debris with refractile material after disruption, disrupting the desalted culture, optionally adding material to the disruptate to create a density or viscosity gradient and separating the refractile material from the cellular debris by high-speed centrifugation. Preferably the salt removal step is carried out by diafiltration and the heterologous protein comprises recombinant M-CSF, IL-2 or IFN-.beta..

600132

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