Method for assaying 1,5-anhydroglucitol and kit therefor

Details Method for assaying 1,5-anhydroglucitol and kit therefor Method for assaying 1,5-anhydroglucitol and kit therefor

C - Chemistry, Metallurgy

12

Q

150/15.1, 150/3.

C12Q 1/26 (2006.01) G01N 33/66 (2006.01)

Patent

CA 1306172

ABSTRACT OF THE DISCLOSURE This invention relates to a novel method for assaying 1,5-anhydroglucitol, which is expected to serve as a marker for diabetes, and a kit therefor. More particularly, it relates to: (1) a method for assaying 1,5-anhydroglucitol, which comprises selectively removing sugars from a specimen containing 1,5- anhydroglucitol; allowing pyranose oxidase or L-sorbose oxidase to act on 1,5- anhydroglucitol contained in the sample thus obtained in the presence of oxygen; and determining 1,5-anhydroglucitol from the amount of the hydrogen peroxide thus formed; and (2) a reagent kit for assaying 1,5-anhydro- glucitol which comprises an agent for removing sugars, a reagent for detecting hydrogen peroxide and an enzyme for oxidizing 1,5-anhydroglucitol.

547332

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