Carbonic anhydrase inhibitor-tagged nucleic acid probes

Details Carbonic anhydrase inhibitor-tagged nucleic acid probes Carbonic anhydrase inhibitor-tagged nucleic acid probes

C - Chemistry, Metallurgy

12

Q

260/301, 167/45,

C12Q 1/68 (2006.01) C07H 19/10 (2006.01) C07H 19/20 (2006.01) C12Q 1/70 (2006.01) G01N 33/532 (2006.01) G01N 33/535 (2006.01)

Patent

CA 1294899

ABSTRACT CARBONIC ANHYDRASE INHIBITOR-TAGGED NUCLEIC ACID PROBES Nucleic acid hybridization probes are provided which comprise nucleoside bases or terminal nucleotide phosphates chemically linked to aromatic sulfonamide inhibitors of carbonic anhydrase. Methods of preparing probes of the invention, intermediates used in such methods, and methods of using the probes of the invention in hybridization assays are also provided. A probe of the invention is detected by binding to it a reporter group, such as a homopolymer or heteropolymer of enzymes, which includes a carbonic anhydrase which binds to the inhibitor linked to the probe, and then detecting the bound reporter group, as by production of a fluorescent or colored product in a reaction catalyzed by an enzyme component of the reporter group. Also provided are enzyme immunoassays wherein detection of antibody is by a process which comprises a chemical reaction catalyzed by a carbonic anhydrase.

510835

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