A - Human Necessities – 61 – K
Patent
A - Human Necessities
61
K
167/129, 167/139
A61K 47/48 (2006.01) A61K 49/00 (2006.01) C07K 15/12 (1990.01) C07K 15/28 (1990.01)
Patent
CA 1336496
Method for producing an immunoconjugate comprising the steps of reacting a toxin or protein with a heterobifunc- tional reagent having the following general formula: Image where R1 is: -(CH2)n- or Image or Image where n = 1 to 10; and where R2 is selected from the group consisting of o- and p-nitrophenyl, 2-chloro-4-nitrophenyl, cyanomethyl, 2-mercapto- pyridyl, hydroxybenztriazole, N-hydroxysuccinimide, trichloro- phenyl, tetrafluorophenyl, 2-fluorophenyl, 4-fluorophenyl, 2,4-difluorophenyl, o-nitro-p-sulfophenyl, N-hydroxyphthali- mide, N,N-diethylamino, N-hydroxypyrrolidone, tetrafluorothio- phenyl, and 2,3,5,6-tetrafluorophenyl, under reactive condi- tions, thereby forming a derivatized toxin or protein. The derivatized toxin or protein is separated from the reaction mixture and combined with an antibody or antibody fragment under reactive conditions, such that at least one native disulfide bond is reduced to produce at least one thiol group, capable of forming a thioether bond between said thiol group and the maleimide group of said derivatized toxin, or protein thereby forming an immunoconjugate. The preferred heterobifunc- tional linking group is succinimidyl 4-(N-maleimidomethyl)- cyclohexane-1-carboxylate (SMCC).
576911
Gowling Lafleur Henderson Llp
Neorx Corporation
LandOfFree
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