Expression and secretion of heterologous proteins by...

Details Expression and secretion of heterologous proteins by... Expression and secretion of heterologous proteins by...

C - Chemistry, Metallurgy

12

N

195/129, 530/13,

C12N 15/52 (2006.01) C07K 14/47 (2006.01) C07K 19/00 (2006.01) C12N 9/60 (2006.01) C12N 9/64 (2006.01) C12N 15/62 (2006.01) C12N 15/81 (2006.01) C12N 15/90 (2006.01)

Patent

CA 1340837

Sequencing of the XPR2 and LEU2 genes of Yarrowia lipolytica, recombinant Yarrowia lipolytica cloning vehicles comprising heterologous DNA coding for the expression of mammalian protein and other polypeptides, including plasmids suited for transformation of Y. lipolytica hosts and incorporating a regulon homologous to the host in its untransformed state, and secretion signals in reading phase with the protein coding sequence for the heterologous gene; integrative expression vectors using the XPR2 gene promoter, alkaline protease pre-proregion and XPR2 terminator region and those having the LEU2 promoter and alkaline protease secretory signal sequences capable, in a transformed Y. lipolytica cell culture, of expressing and secreting a heterologous protein outside the cell; Y. lipolytica transformants comprising said vectors and plasmids; methods for preparing vectors to direct secretion of specific heterelogous proteins coded for by genes, cDNA or synthetic DNA in Y. lipolytica in their mature, functional state.

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