C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/15.5
C12Q 1/52 (2006.01)
Patent
CA 1286960
ABSTRACT "METHOD FOR DETECTION OF PERIODONTAL DISEASE" Disclosed are improvements in methods for determining the presence of suprathreshold amounts of aspartate aminotransferase in an oral fluid sample by (1) incubation of aspartate aminotransferase substrate materials in a first reaction mixture with the sample to form a first reaction product and (2) detection of reac- tion product therein by incubation with an indicator substance in a second reaction mixture wherein a second colored reaction product is formed. The improvement comprises (a) utilizing a diazonium dyestuff as the indicator substance in the second reaction and (b) modifying reaction conditions within the first and second reaction mixtures such that no visually detect- able colored reaction product will be formed in the second reaction mixture by subthreshold level of aspartate aminotransferase but wherein a visually detectable color reaction product will be formed in the reaction mixture when a selected suprathreshold concen- tration of aspartate aminotransferase is present in the first reaction mixture.
532102
Babler Scott D.
Baram Peter
Rudolph Paul H.
Waxman Frank J.
Ridout & Maybee Llp
Xytronyx Inc.
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