Method of concentrating and measuring unicellular organisms

Details Method of concentrating and measuring unicellular organisms Method of concentrating and measuring unicellular organisms

C - Chemistry, Metallurgy

12

Q

150/16.4, 73/51.

C12Q 1/06 (2006.01) C12Q 1/68 (2006.01) G01N 21/64 (2006.01)

Patent

CA 1201963

Method of Concentrating and Measuring Unicellular Organisms Abstract A method of concentrating and measuring unicellular organism content of a sample is described. The unicellu- lar organism containing sample is first filtered through a filter membrane impermeable to the organism to con- centrate and collect the cells on the filter membrane. The membrane containing the concentrated cells is then sequentially treated (1) with a lysing agent to lyse the cells releasing the adenosine triphosphate in the cells and (2) with a luminescent reagent which reacts with the released adenosine triphosphate contained on the filter to produce light. Without significant effect by the filter membrane, the light produced on the mem- brane is then measured on a luminometer. Not only does this method provide an accurate way of detecting very minute quantitites of cells, but it provides a rela- tively quick and easy method of concentrating and measuring cells in large volumes of biological or industrial liquid samples which contain low levels of organisms.

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