Method for separating rennet components

Details Method for separating rennet components Method for separating rennet components

C - Chemistry, Metallurgy

12

N

195/36

C12N 9/64 (2006.01) A23C 19/04 (2006.01)

Patent

CA 1286620

ABSTRACT OF THE DISCLOSURE substantially pure chymosin is separated from rennet extracts by contacting a liquid extract with an anionic exchange medium, preferably a diethylaminoethyl (DEAE) cellulose resin packed in a liquid chromatographic column, either as a static bed or stirred bed, The rennet extract and resin are conditioned to that the resin selectively binds pepsin and chymosin passes through the resin bed and is recovered from the liquid extract from the column. The bound pepsin is periodically removed from the resin by passing a suitable eluant through the resin bed.

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