Method and kit for testing for the presence of catalase in milk

Details Method and kit for testing for the presence of catalase in milk Method and kit for testing for the presence of catalase in milk

C - Chemistry, Metallurgy

12

Q

150/15.1, 150/3.

C12Q 1/37 (2006.01) C12Q 1/30 (2006.01) C12Q 1/34 (2006.01)

Patent

CA 1309325

A METHOD AND KIT FOR TESTING FOR THE PRESENCE OF CATALASE IN MILK ABSTRACT OF THE DISCLOSURE The invention provides a method for testing for the presence of catalase in a milk sample comprising combining a milk sample with a substantially catalase-free alkaline protease enzyme enriched detergent to disrupt catalase containing somatic cells present therein and release active catalase therefrom in the present of a pH buffering salt having a concentration of at least 20mM in the resulting sample solution and providing the solution with a pH in a range of about 7.0-8.0 and thereafter testing the sample for the presence of catalase, as well as providing a test kit for detecting the presence of catalase in milk comprising a substantially catalase-free alkaline protease enzyme enriched detergent and 20 to 400 micromoles of a pH buffer alone or in combination with any other solute capable of reducing the solubility of oxygen in milk and of providing the resulting milk solution with a pH in the range of about 7.0-8Ø

557067

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