Method for immobilization

C - Chemistry – Metallurgy – 12 – N

Patent

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324/23, 195/35.4

C12N 11/08 (2006.01) C12M 1/40 (2006.01) C12N 11/04 (2006.01) C12Q 1/00 (2006.01) G01N 27/327 (2006.01)

Patent

CA 1319633

ABSTRACT The present invention relates generally to the immobilization or incorporation of polypeptides, especially enzymes or other bioactive polypeptides into polymeric matrixes, especially polyurethane, membranes produced by said polymers, and the utilization of such membranes in biosensors. A preferred type of biosensor is the needle sensor designed for in vivo monitoring of glucose which comprises a core platinum anode coated with an insulating lacquer, the anode is situated inside a stainless steel reference cathode which is insulated from the anode by a layer of epoxy resin. At one end, the tip, the electrode has a detection surface, which is in an acute angle to the general direction of the electrode. At the other end, the base, the electrode is provided with terminals and for the anode and cathode, respectively. The terminals are soldered to leads, respectively, which are connected to instrumentation used when performing measurements.

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