Antibody detection process

Details Antibody detection process Antibody detection process

G - Physics

01

N

167/44

G01N 33/86 (2006.01) G01N 33/555 (2006.01)

Patent

CA 1167751

ABSTRACT A process for the detection of antibodies in a test sample is described, This process involves: preparing an essentially isotonic and low ionic strength suspension comprising said sample and erythrocytes is net negatively charged form, which suspension contains a sensitization effective amount of ethylenediamine tetra-acetate; maintaining said suspension for at least 30 seconds; combining said suspension with an amount of solution of polymer effective for agglutination of said erythrocytes; separating the resultant agglutinates of polymer and erythrocytes from supernatant of said suspension; dispersing said agglutinates in a hypertonic salt solution having an essentially neutral pH; and monitoring the resuspended agglutinates for the presence or absence of antibodies. This process permits rapid detection of antibodies with great ease. Their presence is indicated by persistence of the agglutinates in the dispersion. The antigen which reacts with antibody may be native to the erythrocytes, or may be unrelated to erythrocites but artificially coupled to those cells. Quantitative analysis is likewise possible because their dissociation is inversely related to antibody concentration.

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